Dosage & Reconstitution Guide
CJC-1295 No DAC + Ipamorelin Dosage & Reconstitution Guide
A clear educational guide covering CJC-1295 No DAC + Ipamorelin blend reconstitution, syringe unit conversions, gradual protocol examples, supplies, storage, injection technique, GH/IGF-1 research notes and safety considerations.
Quickstart Highlights
- Blend: CJC-1295 No DAC + Ipamorelin
- Common vial size: 10mg total blend
- Blend ratio: commonly 5mg CJC-1295 No DAC + 5mg Ipamorelin
- Example reconstitution: 3.0mL bacteriostatic water
- Total concentration: approximately 3.33mg/mL
- Each peptide concentration: approximately 1.67mg/mL
- U-100 syringe maths: 1 unit = 0.01mL = approximately 33.3mcg total blend
- Common research range discussed: 100-300mcg of each peptide daily
- Storage after mixing: refrigerate at 2-8°C and avoid freeze-thaw cycles
What is CJC-1295 No DAC + Ipamorelin?
CJC-1295 No DAC + Ipamorelin is a growth hormone secretagogue blend commonly discussed in research for supporting pulsatile growth hormone release through two complementary pathways.
CJC-1295 No DAC is a modified GHRH analogue, while Ipamorelin is a selective growth hormone secretagogue. The blend is often discussed in relation to GH and IGF-1 signalling, body composition research, recovery research and sleep-related hormone release patterns.
Reconstitution Example
The example below is based on a 10mg total blend vial mixed with 3.0mL bacteriostatic water.
Reconstitution Maths
10mg total blend ÷ 3.0mL = 3.33mg/mL total blend
3.33mg/mL = 3,333mcg/mL total blend
Each peptide = approximately 1.67mg/mL
1 unit on a U-100 syringe = 0.01mL
3,333mcg/mL × 0.01mL = approximately 33.3mcg total blend per unit
Unit Conversion Table
Standard Gradual Research Example
Frequency and Timing Notes
The source protocol discusses once-daily subcutaneous administration, typically before bed or upon waking. The aim is to align with natural growth hormone pulse patterns while keeping the schedule simple.
The key practical points are accurate measurement, consistent timing, careful site rotation and tracking of any observations.
Reconstitution Steps
- Confirm the total blend size and ratio before calculating concentration.
- Draw 3.0mL bacteriostatic water using sterile technique.
- Inject the bacteriostatic water slowly down the inside wall of the vial.
- Avoid blasting the powder directly and avoid creating foam.
- Gently swirl or roll the vial until dissolved.
- Do not shake.
- Label the vial with the reconstitution date and concentration.
- Store refrigerated at 2-8°C, protected from light.
Supplies Needed
CJC-1295 No DAC + Ipamorelin Vials
- 8-week example: approximately 3 × 10mg blend vials.
- 12-week example: approximately 4 × 10mg blend vials.
- 16-week example: approximately 5 × 10mg blend vials.
Insulin Syringes
- Daily example: 7 syringes per week.
- 8 weeks: approximately 56 syringes.
- 12 weeks: approximately 84 syringes.
- 16 weeks: approximately 112 syringes.
- Practical note: 30-unit or 50-unit syringes may be preferred for low-volume measurements.
Bacteriostatic Water
- Per 10mg blend vial: 3.0mL bacteriostatic water.
- 8-week example: 3 vials × 3.0mL = 9mL total, so 1 × 10mL bottle may be enough.
- 12-week example: 4 vials × 3.0mL = 12mL total, so 2 × 10mL bottles may be needed.
- 16-week example: 5 vials × 3.0mL = 15mL total, so 2 × 10mL bottles may be needed.
Alcohol Swabs
- Per administration: one swab for the vial stopper and one for the injection site.
- Per week: approximately 14 swabs based on daily administration.
- 8 weeks: around 112 swabs, so 2 × 100-count boxes may be needed.
- 12 weeks: around 168 swabs, so 2 × 100-count boxes may be needed.
- 16 weeks: around 224 swabs, so 3 × 100-count boxes may be needed.
Protocol Overview
- Goal discussed in research: pulsatile GH release through GHRH and GHS pathway stimulation.
- Schedule example: once-daily subcutaneous administration.
- Common amount discussed: 100-300mcg of each peptide daily with gradual titration.
- Example reconstitution: 3.0mL bacteriostatic water per 10mg blend vial.
- Concentration: approximately 3.33mg/mL total blend.
- Cycle length: 8-12 weeks is commonly discussed, with optional extension to 16 weeks.
- Timing: commonly before bed or upon waking.
- Storage: lyophilised frozen, reconstituted refrigerated and not frozen after mixing.
Example Dosing Protocol
- Start: 100mcg of each peptide daily.
- Titration: increase by around 50mcg every 1-2 weeks as tolerated.
- Target example: 200-300mcg of each peptide daily by Weeks 5-12.
- Frequency: once daily in the source protocol.
- Timing: before bed or upon waking is commonly discussed.
How CJC-1295 No DAC + Ipamorelin Works
CJC-1295 No DAC is a modified GHRH analogue that stimulates pulsatile growth hormone release from the pituitary. It is discussed for dose-dependent increases in GH and IGF-1.
Ipamorelin is a selective growth hormone secretagogue that can trigger a rapid GH pulse. It is commonly discussed as being more selective than some older secretagogues, with less effect on ACTH, cortisol or prolactin pathways.
When combined, the two peptides may act on complementary receptor pathways, which is why this blend is often discussed in GH secretagogue research.
Potential Benefits Discussed in Research
- Pulsatile growth hormone release research
- IGF-1 pathway research
- Growth hormone secretagogue research
- Body composition research
- Recovery and sleep-related hormone pathway discussions
- Metabolic adaptation research
- Selective GH release research involving Ipamorelin
- GHRH analogue research involving CJC-1295 No DAC
Reported Side Effects and Limitations
- Flushing
- Headache
- Injection-site redness or irritation
- Increased appetite
- Water retention
- Tingling sensations
- Human data is limited outside specific research contexts
- Not a guaranteed treatment for fatigue, sleep, recovery, fat loss or muscle gain
Storage Instructions
- Lyophilised powder: store frozen at around -20°C in dry, dark conditions.
- After reconstitution: refrigerate at 2-8°C.
- Use window: commonly discussed as around 28 days after reconstitution.
- Do not freeze after mixing: avoid freezing reconstituted solution.
- Protect from light: keep the vial away from unnecessary light exposure.
- Avoid: heat, sunlight, moisture, contamination and repeated freeze-thaw cycles.
- Practical note: allow vials to reach room temperature before opening to reduce condensation uptake.
Injection Technique Notes
This section is general educational information only. It is not personal injection instruction.
- Use a new sterile insulin syringe for each administration.
- Clean the vial stopper and injection site with alcohol and allow both to dry completely.
- Common subcutaneous sites discussed include abdomen, thighs and upper arms.
- Rotate injection sites systematically to reduce local irritation and lipohypertrophy risk.
- Pinch a skinfold and insert into subcutaneous tissue at an appropriate angle.
- Do not aspirate for subcutaneous injections.
- Inject slowly and steadily.
- Wait a few seconds before withdrawing the needle to help reduce leakage.
- Dispose of used sharps safely in a sharps container.
Lifestyle Factors That May Matter
- Sleep: growth hormone is predominantly released during deep sleep, so sleep quality matters.
- Nutrition: a balanced, protein-forward diet may support body composition and recovery goals.
- Training: resistance training and aerobic activity are commonly paired with metabolic and body composition goals.
- Stress management: stress reduction may support hormonal balance, recovery and adherence.
- Tracking: documenting amount, date, site and observations can make tolerance patterns easier to review.
Use the Peptide Calculator
If your vial size, water volume or target amount is different, use the Peptide Hub Australia calculator to work out concentration, mL and syringe units.
Calculate CJC-1295 No DAC + Ipamorelin Units
Enter your vial size, BAC water amount and target amount to calculate concentration and syringe units.
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Frequently Asked Questions
How much BAC water do you add to a 10mg CJC-1295 No DAC + Ipamorelin blend?
A common educational example is 3.0mL bacteriostatic water added to a 10mg blend vial, creating a total concentration of approximately 3.33mg/mL.
How many units is 100mcg of each peptide?
If a 10mg blend vial is mixed with 3.0mL, 100mcg of each peptide is approximately 3 units on a U-100 insulin syringe, equal to around 0.03mL.
How many units is 200mcg of each peptide?
If a 10mg blend vial is mixed with 3.0mL, 200mcg of each peptide is approximately 6 units on a U-100 insulin syringe, equal to around 0.06mL.
How many units is 300mcg of each peptide?
If a 10mg blend vial is mixed with 3.0mL, 300mcg of each peptide is approximately 9 units on a U-100 insulin syringe, equal to around 0.09mL.
Why are the unit amounts so small?
This blend uses low microgram amounts per peptide. Because of that, the measured volume can be under 10 units, which may be harder to read on larger 1mL syringes.
When is CJC-1295 No DAC + Ipamorelin commonly discussed as being used?
The source protocol discusses once-daily use, typically before bed or upon waking.
Is this a personal dosing recommendation?
No. This page is an educational reconstitution and measurement guide only. It is not medical advice or a personal protocol.
